image001image002The Cytokine and Phagocyte Biology Lab

 

Index

*                         Who and where are we?

*                         Teaching

*                         Research interest

*                         Personnel

*                         Publications

*                         Research grants

*                         Positions available:

Who and where are we?

Academic staff/Senior Scientists:

Prof. Victoriano Mulero

Prof. Alfonsa Garcia-Ayala

Prof. Jose Meseguer

Dr. Maria P. Sepulcre

Address:

Department of Cell Biology and Histology. Faculty of Biology.

University of Murcia. 30100 Murcia. SPAIN.

Contact us:

V. Mulero: vmulero@um.es, +34 868 887581

A. Garcia-Ayala: agayala@um.es, +34 868 884968

J. Meseguer: meseguer@um.es, +34 868 884965

M.P. Sepulcre: mpsepul@um.es, +34 868 883938

FAX: +34 868 883963

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Teaching

BSc courses:

Biology degree
 
Embriology
Cell Biology
 
 
Biotechnology degree
 
Cell Biology
Basic Cell Biology Techniques
Advanced Cell Biology Techniques
Tissue engineering 
Evolutionary immunology
Animal cell culture
Biotechnological applications of antibodies 
 

MSc and PhD courses:

Techniques and research in Biomedicine (Quality Mention)
 
 Cell Biology Techniques (1st year)
 Gene transcription and Acquisition of Cell Function (1st year)
 Phylogeny of Cytokines (Research line)
 
Fisheries and aquaculture
 
 Fish Immunity (1st year)
 The Immune Response of Fish (Research line)
 
Molecular Biology and Biotechnology (Quality Mention)
 
 Localization of Biomolecules (1st year)
 Evolution of Immunity in Vertebrates (Research line)

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Research interest

Phylogeny of immunity:

 Cytokines: functional characterisation of IL-1b, TNFa and IFNs.
 Pattern recognition receptors: functional characterization of TLRs
 Prostaglandins: role of prostaglandins, (PGE2, PGD2 and PGJ2) in the regulation of inflammation 
 Phagocytes: role of granulocytes and macrophages in the immune response 
 Gut immunity: functional characterization of mast cells
 Reproductive immunology: role of leukocytes and cytokines in spermatogenesis

Aquaculture:

 Development of oral immunostimulants and adjuvants
 Development of oral vaccines

Biomedicine:

 TNFR signaling in hematopoiesis, inflammation and cancer
 In vivo imaging of inflammation and tumour development
 Identification of new pharmacological compounds to treat chronic inflammation and cancer by high throughput screening 
 

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Personnel

Postdocs

Dr. Isabel Cabas  icabas@um.es

 Effects of estrogens on innate immunity

Dr. Sergio Candel scandel@um.es

 Role of TNFa signalling in neutrophils development and functions using the zebrafish

Dr. Jorge Galindo-Villegas jorge-galindo@um.es

 Oral immunostimulation and vaccination
 Interactions between microbiota and immunity 

Dr. Chiara Gabellini donegal77@gmail.com  

 Modeling the impact of the anti-apoptotic protein bcl-xL in melanoma angiogenesis and progression using the zebrafish 
 
Dr. Diana Garcia-Moreno dianagm@um.es 
 Epigenetic regulation of inflammation in zebrafish
 Implementation of site directed mutagenesis by Zinc-Finger nucleases

Dr. Azucena Lopez-Muñoz malms@um.es

 Molecular and functional characterisation of fish IFNs

Dr. Jana Montero jana@um.es

 Functional characterisation of fish soluble TLR5 and intracellular flagellin signaling
 

PhD students

Elena Gomez  elena.gomez1@um.es

 Modelling the impact of inflammation on melanoma development using the zebrafish.

Nuria E. Gomez-Gonzalez  nuriaesther.gomez@um.es

 Functional characterization of fish mast cells and role of histamine in innate and adaptive immunity.

Victoria Gómez-Abellan vga4@um.es

 Role of prostaglandins in the regulation of fish immunity 

Sofia de Oliveira sloliveira@fm.ul.pt

 Modelling neutrophil migration in vivo using the zebrafish 

Maricarmen Rodenas mariacarmen.rodenas1@um.es

 Effects of endocrine disruptors on immunity

Sylwia Tyrkalska  tyrkalska.sylwia@gmail.com

 Identification of new inflammasome components

 

Technicians

Inmaculada Fuentes ifm@um.es

 Lab manager

Pedro Martinez pedrojmartinez@um.es

 Zebrafish facility manager

 

Former lab's members

Dr. Pablo Pelegrin pablo.pelegrin@ffis.es

Unidad de Cirugia Experimental, Hospital Universitario Virgen de la Arrixaca. 30120 El Palmar. Murcia. Spain.

Dr. Jesus Garcia-Castillo jesus.garciac@umh.es

Instituto de Neurociencias, Campus de Sant Joan, Avd Santiago Ramon y Cajal s/n, 03550 Alicante, Spain.

Dr. Gloria Lopez-Castejon Gloria.Lopez-Castejon@manchester.ac.uk

Faculty of Life Sciences, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK

Dr. Elena Chaves-Pozo elena.chaves@mu.ieo.es  

Centro de Investigación en Sanidad Animal (CISA-INIA), Carretera de Algete a El Casar km. 8.1, Valdeolmos, 28130 Madrid, Spain.

Dr. Ivan Mulero  ivan.mulero@um.es

Dr. Jaya Kumari  jaya_kumari2005@rediffmail.com

Dr. Francisco J. Roca fjroca@u.washington.edu

Department of Microbiology, University of Washington, Box 357242, Seattle, WA 98195, USA.

Dr. Patricia Castillo-Briceño  patricia.castillo-briceno@univmed.fr

Institut de Biologie du Développement de Marseille Luminy, UMR 6216, CNRS-Université de la Méditerranée, Case 907, 13288 Marseille Cedex 09, France.

Dr. Iciar Muñoz  iciarmunoz@um.es

Dr. Sergio Liarte  sdll1@um.es

Nicolas Gonzalez-Tejero ngm5@um.es

Ana Montalban  ana.montalban-arques@medunigraz.at

Zentrum für Medizinische Forschung (ZMF)
Stiftingtalstraße 24
A-8010 Graz
 

Dr. Raquel Espin  respin@um.es

Department of Cellular and Molecular Medicine and Section of Cell and Developmental Biology, University of California at San Diego, La Jolla, California, USA.

Dr. Diego Angosto  dangosto@um.es

Dr. Erick Garcia-Garcia dr.erick.garcia@gmail.com

 

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Selected Publications (Click here for a full list)

MULERO I, SEPULCRE MP, MESEGUER J, GARCIA-AYALA A, MULERO V (2007). Histamine is stored in mast cells of most evolutionarily advanced fish and regulates the fish inflammatory response. Proc Natl Acad Sci USA. 104: 19434-19439. Full text

ROCA FJ, MULERO I, LOPEZ-MUÑOZ A, SEPULCRE MP, MESEGUER J, MULERO V (2008). Evolution of the inflammatory response in vertebrates: fish TNFa is a powerful activator of endothelial cells but hardly activates phagocytes. J Immunol 181:5071-5081. Full text

ALCARAZ-PEREZ F, MULERO V, CAYUELA ML (2008). Application of the Dual-Luciferase Reporter Assay to the Analysis of Promoter Activity in Zebrafish Embryos. BMC Biotechnol 8:81. Full text

SEPULCRE MP, ALCARAZ-PÉREZ F, LÓPEZ-MUÑOZ A, ROCA FJ, MESEGUER J, CAYUELA ML, MULERO V (2009). Evolution of LPS Recognition and Signaling: Fish TLR4 Does Not Recognize LPS and Negatively Regulates NF-kB Activation. J Immunol. 182: 1836-1845 Full text

LÓPEZ-MUÑOZ A, ROCA FJ, MESEGUER J, MULERO V (2009). New insights into the evolution of IFNs: Zebrafish group II IFNs induce a rapid and transient expression of IFN-dependent genes and display powerful antiviral activities. J Immunol 182: 3440-3449. Full text

OLAVARRÍA VH, SEPULCRE MP, FIGUEROA JE, MULERO V. (2010). Prolactin-induced production of ROS and IL-1b in leukocytes from the bony fish gilthead seabream involves Jak/Stat and NF-kB signaling pathways. J Immunol. 185:3873-3883. Full text

LÓPEZ-MUÑOZ A SEPULCRE MP, ROCA FJ, FIGUERAS A, MESEGUER J, MULERO V (2011). Evolutionary conserved pro-inflammatory and antigen presentation functions of zebrafish IFNγ revealed by transcriptomic and functional analysis. Mol. Immunol. 48:1073-1083. Full text

MULERO V, SEPULCRE MP, RAINGER GE, BUCKLEY CD (2011). Neutrophils live on a two way street. J Leukoc Biol 89:645-647. Full text.

OLAVARRÍA VH, FIGUEROA JE, MULERO V. (2012). Prolactin-induced activation of phagocyte NADPH oxidase in the teleost fish gilthead seabream involves the phosphorylation of p47phox by protein kinase C. Dev Comp Immunol. 36:216-221. Full text.

CABAS I, LIARTE S, GARCÍA-ALCÁZAR A, MESEGUER J, MULERO V, GARCÍA-AYALA A. (2012). 17α-Ethynylestradiol alters the immune response of the teleost gilthead seabream (Sparus aurata L.) both in vivo and in vitro. Dev Comp Immunol. 36:547-556 Full text.

LÓPEZ-MUÑOZ A SEPULCRE MP, GARCÍA-MORENO D, SEPULCRE MP, FUENTES I, BÉJAR J, MANCHADO M, ALVAREZ MC, MESEGUER J, MULERO V (2012). Viral nervous necrosis virus persistently replicates in the central nervous system of asymptomatic gilthead seabream and promotes a transient inflammatory response followed by the infiltration of IgM+ B lymphocytes. Dev Comp Immunol 7:429-437. Full text.

ANGOSTO D, LÓPEZ-CASTEJÓN G, LÓPEZ-MUÑOZ A SEPULCRE MP, ARIZCUN M, MESEGUER J, MULERO V (2012). Evolution of inflammasome functions in vertebrates: Inflammasome and caspase-1 trigger fish macrophage cell death but are dispensable for the processing of IL-1β. Innate Immun 18:815-824. Full text.

AGUILA S, CASTILLO-BRICEÑO P, SÁNCHEZ M, CABAS I, GARCÍA-ALCÁZAR A, MESEGUER J, MULERO V, GARCÍA-AYALA A. (2012). Specific and non-overlapping functions of testosterone and 11-ketotestosterone in the regulation of professional phagocyte responses in the teleost fish gilthead seabream. Mol Immunol. 53:218-226. Full text.

GALINDO-VILLEGAS J, GARCÍA-MORENO D, DE OLIVERIA S, MESEGUER J, MULERO V (2012). Regulation of immunity and disease resistance by commensal microbes and chromatin modifications during zebrafish development. Proc Natl Acad Sci USA 109: E2605-E2614. Full text. Full text Author Summary.

COMPAN V, BAROJA-MAZO A, LÓPEZ-CASTEJÓN G, GOMEZ AI, MARTÍNEZ CM, ANGOSTO D, MONTERO MT, HERRANZ AS, BAZÁN E, REIMERS D, MULERO V, PELEGRÍN P (2012). Cell volume regulation modulates NLRP3 inflammasome activation. Immunity 37:487-500. Full text.

ESPÍN R, ROCA FJ, CANDEL S, SEPULCRE MP, GONZÁLEZ-ROSA JM, ALCARAZ-PÉREZ F, MESEGUER J, CAYUELA ML, MERCADER N, MULERO V (2013). TNF receptors regulate vascular homeostasis through a caspase-8, caspase-2 and P53 apoptotic program that bypasses caspase-3. Dis Model Mech 6:383-396. Full text.

ANGOSTO D, LÓPEZ-CASTEJÓN G, MULERO V, PELEGRÍN P (2013). Response to Boyle et al.: Cell Swelling and the NLRP3 Inflammasome. Immunity 38:400-401. Full text.

DE OLIVEIRA S, REYES-ALDASORO CC, CANDEL S, RENSHAW SA, MULERO V, CALADO A. (2013). Cxcl8 (IL-8) mediates neutrophil recruitment and behavior in the zebrafish inflammatory response. J Immunol 190:4349-4359. Full text.

OLAVARRÍA VH, FIGUEROA JE, MULERO V (2013). Induction of genes encoding NADPH oxidase components and activation of interferon regulatory factor-1 by prolactin in fish macrophages. Innate Immun. 19:644-654. Full text.

GARCÍA-GARCIA E, GALINDO-VILLEGAS J, MULERO V. (2013). Mucosal immunity in the gut: the non-vertebrate perspective. Dev Comp Immunol 40:278-288. Full text.

MUÑOZ I, SEPULCRE MP, MESEGUER J, MULERO V (2013). Molecular cloning, phylogenetic analysis and functional characterization of soluble Toll-like receptor 5 in gilthead seabream, Sparus aurata. Fish Shellfish Immunol 35:36-45. Full text.

ANCHELIN M, ALCARAZ-PÉREZ F, MARTÍNEZ CM, BERNABÉ-GARCÍA M, MULERO V, CAYUELA ML (2013). Premature aging in telomerase-deficient zebrafish. Dis Model Mech. 6:1101-1112. Full text.

GALINDO-VILLEGAS J, MULERO I, GARCÍA-ALCAZAR A, MUÑOZ I, PEÑALVER-MELLADO M, STREITENBERGER S, SCAPIGLIATI G, MESEGUER J, MULERO V. (2013) Recombinant TNFα as oral vaccine adjuvant protects European sea bass against vibriosis: Insights into the role of the CCL25/CCR9 axis. Fish Shellfish Immunol 35:1260-1271. Full text.

ANGOSTO D, MONTERO J, LÓPEZ-MUÑOZ A, ALCARAZ-PÉREZ F, BIRD S, SARROPOULOU E, ABELLÁN E, MESEGUER J, SEPULCRE MP, MULERO V (2013). Identification and functional characterization of a new IL-1 family member, IL-1Fm2, in most evolutionarily advanced fish.. Innate Immun (in press). Full text.

CABAS I, RODENAS MC, ABELLÁN E, MESEGUER J, MULERO V, GARCÍA-AYALA A. (2013). Estrogen signaling through the g protein-coupled estrogen receptor regulates granulocyte activation in fish. J Immunol 191:4628-4639. Full text.

CABAS I, CHAVES-POZO E, GARCÍA-ALCÁZAR A, MESEGUER J, MULERO V, GARCÍA-AYALA A (2013). The effect of 17α-ethynylestradiol on steroidogenesis and gonadal cytokine gene expression is related to the reproductive stage in marine hermaphrodite fish. Mar Drugs. 11:4973-4992. Full text.

MUÑOZ I, SEPULCRE MP, MESEGUER J, MULERO V. (2014). Toll-like receptor 22 of gilthead seabream, Sparus aurata: Molecular cloning, expression profiles and post-transcriptional regulation. Dev Comp Immunol. 44:173-179. Full text.

SÁNCHEZ-HERNÁNDEZ M, ARIZCUN M, GARCÍA-ALCÁZAR A, SARROPOULOU E, MULERO V, GARCÍA-AYALA A. (2014). Fish granulocytes express a constitutively active androgen receptor variant. Dev Comp Immunol. 45:115-122. Full text.

ALCARAZ-PEREZ F, GARCIA-CASTILLO J, GARCIA-MORENO D, LOPEZ-MUNOZ A, ANCHELIN M, ANGOSTO D, ZON LI, MULERO V*, CAYUELA ML* (2014). A non-canonical function of telomerase RNA in the regulation of developmental myelopoiesis in zebrafish". Nat Commun 5:3228. Full text.

CANDEL S, DE OLIVEIRA S, LÓPEZ-MUÑOZ A, GARCÍA-MORENO D, ESPÍN-PALAZÓN R, TYRKALSKA SD, CAYUELA ML, RENSHAW SA, CORBALÁN R, VIDAL-ABARCA I, TSAI H-J, MESEGUER J, SEPULCRE MP, MULERO V. (2014). Tnfa Signaling Through Tnfr2 Protects Skin Against Oxidative Stress-Induced Inflammation. Plos Biol 12: e1001855. Full text.

DE OLIVEIRA S, LÓPEZ-MUÑOZ, A, CANDEL S, PELEGRIN P, CALADO A. MULERO V (2014). ATP modulates acute inflammation in vivo through dual oxidase 1-derived H2O2 production and NF-κB activation. J Immunol (in press). Full text

Research grants

Our research is funded by:

 

Ministerio de Economía y Competitividad - Gobierno de España

Secretaría de Estado de Investigación, Desarrollo e Innovación - Ministerio de Economía y Competitividad - Gobierno de España

 

TargetFish

FishforPharma Logo

 

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Positions available

No positions are available at this moment

Please contact us: vmulero@um.es

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Updated: May 2014