Publicaciones

Fisiología de la Reproducción
Universidad de Murcia Departamento de Fisiología
Facultad de Veterinaria
Campus Universitario de Espinardo. 30100 Murcia. España

Publicaciones científicas del grupo / Selected publications

Artículos en revistas incluidas en Science Citation Index (SCI) del Institute for Scientific Information (ISI).

Full paper in Science Citation Index (SCI) from Institute for Scientific Information (ISI)

2010

COY P, AVILES M. What controls polyspermy in mammals, the oviduct or the oocyte? Biol Rev Camb Philos Soc. 2009 Dec 23. [Epub ahead of print]
COY P, LLOYD R, ROMAR R, SATAKE N, MATAS C, GADEA J, HOLT WV. Effects of porcine pre-ovulatory oviductal fluid on boar sperm function. Theriogenology (en prensa)
GADEA J, GARCÍA-VAZQUEZ FA. Aplicaciones de los cerdos transgénicos en biomedicina y producción animal. ITEA INFORMACION TECNICA ECONOMICA AGRARIA. 2010. 106(1):15-30.
GADEA J, GARCÍA-VAZQUEZ FA. Métodos de generación de cerdos transgénicos. ITEA INFORMACION TECNICA ECONOMICA AGRARIA. 2010. 106(1):31-45.
GARCÍA-VAZQUEZ FA, RUIZ S, GRULLÓN LA, DE ONDIZ A, GUTIERREZ-ADAN A, GADEA J. Transgénesis mediada por espermatozoides en la especie porcina: efecto de la presencia de ADN exógeno sobre la calidad seminal y evaluacion de la producción in vivo de embriones transgénicos. Revista científica de la Facultad de Ciencias Veterinarias de la Universidad del Zulia. 2010. 20:81-88.
MARTINEZ-SOTO JC, HOURCADE JD, GUTIÉRREZ-ADÁN A, LANDERAS JL, GADEA J Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoa. Asian Journal of Andrology. 2010 Feb 22. [Epub ahead of print]. .
ROMAR R, DE SANTIS T, PAPILLIER P, PERREAU C, THELIE A, DELL’AQUILA ME, MERMILLOD P, DALBIES-TRAN R. Expression of maternal transcripts regulation during bovine oocyte in vitro maturation is affected by donor age. Reproduction in Domestic Animals (en prensa).

2009

CANOVAS S, ROMAR R, GRULLON LA, AVILES M, COY P. Pre-fertilization zona pellucida hardening by different cross-linkers affects in vitro fertilization in pigs and cattle and improves embryo production in pigs. Reproduction. 2009 May;137(5):803-12

COY P, GADEA J, RATH D, HUNTER RHF. Differing sperm ability to penetrate the oocyte in vivo and in vitro as revealed using colloidal preparations. Theriogenology. 72 (2009) 1171–1179.

GARCÍA-ROSELLÓ E, GARCÍA-MENGUAL E, COY P, ALFONSO J, SILVESTRE MA. Intracytoplasmic Sperm Injection in Livestock Species: An Update. Reprod. Domest. Anim. 2009. 44, 143–151.

GARCÍA-VAZQUEZ FA, GUTIERREZ-ADAN A, GADEA J. Evaluación de la unión espermatozoide-ADN exógeno en espermatozoides eyaculados y epididimarios. Archivos de Medicina Veterinaria. 2009. 41.131-138.

GARCÍA-VAZQUEZ FA, GARCIA-ROSELLÓ E, GUTIERREZ-ADAN A, GADEA J. Effect of the sperm treatment on efficiency of porcine transgenic embryo production by ICSI-SMGT. Theriogenology. 2009 Sep 1;72(4):506-18.

LLOYD RE, ROMAR R, MATAS C, GUTIÉRREZ-ADÁN A, HOLT WV, COY P. Effects of oviductal fluid on the development, quality and gene expression of porcine blastocyst produced in vitro. Reproduction. 2009 Apr;137(4):679-87.

2008

CANOVAS S, COY P. Aspectos moleculares de la fecundación: unión y fusión de gametos. Revista de Investigación clínica. 2008. 60 (5):403-413..
CAMPOS I, GOMEZ E, FERNANDEZ-VALENCIA AL, LANDERAS J, GONZALEZ R, COY P, GADEA J. Effects of male and recipient age on the reproductive outcome of an oocyte donation program. J. Assisted Reproduction and Genetics. 2008 Sep-Oct;25(9-10):445-52.
CARRASCO LC, COY P, AVILES M, GADEA J, ROMAR R. Glycosidase determination in bovine oviductal fluid at follicular and luteal phases of the estrous cycle. Reproduction Fertility and Development 2008. 20: 808-817..

CARRASCO LC, ROMAR R, AVILES M, GADEA J, COY P. Determination of glycosidase activity in porcine oviductal fluid at the different phases of the estrous cycle. Reproduction 2008. 136: 833–842.

COY P, GRULLÓN LA, CANOVAS S, ROMAR R, AVILES M, MATAS C. Hardening of the zona pellucida of unfertilized eggs can reduce polyspermic fertilization in the pig and cow. Reproduction. 135:19-27. 2008.

COY P, CANOVAS S, ROMAR R, MONDEJAR I, SAAVEDRA MD, GRULLON LA, MATÁS C, AVILES M. Oviduct-specific glycoprotein and heparin modulate sperm-zona pellucida interaction during mammalian fertilization. Proc Natl Acad Sci U S A 105 (41) 15809–15814. 2008 .

GADEA J, GUMBAO D, CÁNOVAS S, GARCÍA-VÁZQUEZ FA, GRULLÓN LA, GARDÓN JC. Supplementation Of The Thawing Media With Reduced Glutathione Improves Function And The In Vitro Fertilizing Ability Of Frozen-Thawed Bull Spermatozoa. International Journal of Andrology.; 31:40-49. 2008.

RATH D, SCHUBERTH HJ, COY P, TAYLOR U. Sperm interactions from insemination to fertilisation. Reprod Dom Anim. 43 (Suppl. 5), 2–11. 2008.

2007

CANOVAS, COY P, GOMEZ E. First steps for the development of a functional assay for human sperm using pig oocytes. Journal of Andrology. 2007 Mar-Apr;28(2):273-81
GARCÍA-VÁZQUEZ F, GRULLÓN L, RUIZ S, GUTIÉRREZ-ADÁN A, GADEA J. Espermatozoides como vectores de DNA exógeno: producción de embriones porcinos transgénicos mediante diferentes tratamientos espermáticos. ITEA INFORMACION TECNICA ECONOMICA AGRARIA. 28: 54- 56. 2007.
GARCÍA-VÁZQUEZ F, GUMBAO D, GUTIÉRREZ-ADÁN A, GADEA J. Efecto del tratamiento espermático y el tamaño de adn en la interacción transgén- espermatozoide en la especie porcina. ITEA INFORMACION TECNICA ECONOMICA AGRARIA. 28:51-53. 2007.
MATÁS C, GARCÍA-VÁZQUEZ FA, SANSEGUNDO M, GADEA J, COY P, RUIZ S. Estudio de la capacitación espermática in vitro en espermatozoides eyaculados y epididimarios. ITEA INFORMACION TECNICA ECONOMICA AGRARIA. 28:30-32. 2007.
MATÁS C, MARTÍNEZ-MIRÓ S, DECUADRO G, GADEA J Evaluation of a cushioned method for centrifugation and processing for freezing boar semen. Theriogenology. 2007 Mar 15;67(5):1087-91
VELÁSQUEZ JG, CÁNOVAS S, BARAJAS P, MARCOS J, JIMÉNEZ-MOVILLA M, GUTIÉRREZ GALLEGO R, BALLESTA J, AVILÉS M, COY P. Role of sialic acid in bovine sperm-zona pellucida binding. Molecular Reproduction and Development. 2007 May;74(5):617-28.

2006

Ducci M, Pacchini S, Niccolini A, Gazzano A, Cerri D, Gadea J, Bobowiec R, Martelli F. . Concentrations of carnosine, anserine, L-histidine and 3-methyl histidine in boar spermatozoa and sheep milk by a modified HPLC method. Pol J Vet Sci. 2006;9(3):159-63.
Garcia-Rosello E, Coy P, Garcia Vazquez FA, Ruiz S, Matas C. Analysis of different factors influencing the Intracytoplasmic sper Injection (ICSI) yield in pigs. Theriogenology 2006 Nov;66(8):1857-1865
García-Roselló E, Matás C, Canovas S, Moreira PN, Gadea J, Coy P. Influence of sperm pretreatment on the efficiency of Intracytoplasmic Sperm Injection (ICSI) in pigs. J. Androl . 27(3):268-275. 2006.
Rath D, Töpfer-Petersen E, Michelmann H-W, Schwartz P, von Witzendorff D, Ebeling S, Ekhlasi-Hundrieser M, Piehler E, Petrunkina A, Romar R. Structural, biochemical and functional aspects of sperm-oocyte interactions in pigs. In Control of Pig Reproduction VII. Ed.: Ashworth CJ & Kraeling RR. Nottinghan University Press. ISBN 1-904761-33-X. 2006. pp: 317-330.
Romar R, Funahashi H. In vitro maturation and fertilization of porcine oocytes pretreated 48 h with roscovitine, an inhibitor of p34cdc2/cyclin B kinase. Animal Reproduction Science. 92(3-4):321-333. 2006.

2005

Coy P, Romar R, RUIZ S, CANOVAS S, Gadea J, García-Vazquez F, Matas C. Birth of piglets after transferring of in vitro produced embryos pre-matured with R-roscovitine. Reproduction 129(6): 747-755. 2005.
Coy P, Romar R, Payton RR, McCann L, Saxton AM, Edwards JL. Maintenance of meiotic arrest in bovine oocytes using the S-enantiomer of roscovitine: Effects on maturation, fertilization and subsequent embryo development in vitro. Reproduction; 129 (1): 19-26. 2005.
Gadea J. Sperm factors related to in vitro and in vivo porcine fertility. Theriogenology 63(2):431-444. 2005.
Gadea J, García-Vazquez FA, Matás C, Gardón JC, Cánovas S, Gumbao D. Cooling and freezing of boar spermatozoa: Supplementation of the freezing media with reduced glutathione preserves sperm function. J. Androl. 26(3): 396-404. 2005.
Gadea J, Gumbao D, Matás C, Romar R. Supplementation of the thawing media with reduced glutathione improves function and the in vitro fertilizing ability of boar spermatozoa after cryopreservation. J. Androl. 26(6): 749-756. 2005.
Marco-Jiménez F, Puchades S, Gadea J, Vicente JS, Viudes-de-Castro MP. Freezing of Guirra ram spermatozoa. Effect of semen collection method on semen quality. Theriogenology.64:1756-1765. 2005
ROMAR R, COY P, GADEA J, RATH D. Effect of oviductal and cumulus cells on zona pellucida and cortical granules of porcine oocytes fertilized in vitro with epididymal spermatozoa. Anim Reprod Sci 85 (3-4): 287-300 (2005).

2004

Funahashi H, Romar R. Reduction of the incidence of polyspermic penetration into porcine oocytes by pretreatment of fresh spermatozoa with adenosine and a transient co-incubation of the gametes to caffeine.Reproduction 128: 789-800. (2004).
GADEA J, SELLÉS E, MARCO MA, COY P, MATÁS C, ROMAR R, RUIZ S. Decrease in glutatione content in boar sperm alter cryopreservation. Effect of the addition of reduced glutathione to the freezing and thawing extenders. Theriogenology 62:690-701. 2004.
GADEA J, SELLES E, MARCO MA. The importance of porcine seminal analysis on the fertility under commercial conditions. Reprod. Domest. Anim. 39, 303–308. 2004.
PAYTON BP, ROMAR R, COY P, SAXTON AM, LAWRENCE JL, EDWARDS JL Susceptibility of Bovine Germinal Vesicle-Stage Oocytes from Antral Follicles to Direct Effects of Heat Stress In Vitro. Biol Reprod 71, 1303–1308. 2004.

2003

MATÁS C, COY P, ROMAR R, GADEA J, RUIZ S. Effect of sperm preparation method on in vitro fertilisation in the pig. Reproduction 125: 133-141. 2003.
Rath D, Ruiz S, Sieg B. Birth of female piglets after intra-uterine Insemination, employing flowcytometrically sexed boar semen, a case report. Veterinary Record 152: 400-401. 2003.
Romar R, Coy P, Ruiz S, Gadea J, Rath D. Effect of oviductal and cumulus cells on in vitro fertilization and embryo development of porcine oocytes fertilized with epidymal spermatozoa. Theriogenology 59:975-986. 2003.
SELLÉS E, GADEA J, ROMAR R, MATÁS C, RUIZ S. Analysis of in vitro fertilizing capacity to evaluate the freezing procedures of boar semen and to predict the subsequent fertility. Reprod Dom Anim 38:66-72. 2003.

2002

COY P, GADEA J, ROMAR R, MATÁS C, GARCÍA E. In vitro fertilization medium affects the acrosome reaction, cortical reaction, zona pellucida hardening, and in vitro development in the pig. Reproduction 124: 279-288. 2002.
COY P, ROMAR R. In vitro production of pig embryos. A point of view. Reproduction Fertility and Development 14:275-286. 2002.

2001

CAMPOS I, COY P, ROMAR R, RUIZ S, GADEA J. Effect of maturational stage, coincubation and cumulus cells on in vitro penetrability of porcine oocytes. Theriogenology 55: 1489-1500. 2001.
ROMAR R, COY P, CAMPOS I, GADEA J, MATÁS C, RUIZ S. Effect of co-culture with porcine oviductal epithelial cells (POEC) on pig in vitro fertilization. Anim Reprod Sci 68: 85-98. 2001.

<2000

GADEA J, MATÁS C. Sperm factors related to in vitro penetration of porcine oocytes. Theriogenology 54: 1343-1357. 2000.
COY P, RUIZ S, ROMAR R, CAMPOS I, GADEA J. Maturation, fertilization and full development of porcine oocytes matured under different systems. Theriogenology 51: 799-812. 1999.
GADEA J, MATÁS C, LUCAS X. Prediction of porcine semen fertility by homologous in vitro penetration (hIVP) assay. Anim Reprod Sci 54: 95-108. 1998.
VÁZQUEZ JM, MARTÍNEZ E, ROCA J, MATÁS C, BLANCO O. The fertilizing ability assessment of fresh and stored boar semen. Reprod Dom Anim 33: 267-270. 1998.
MARTÍNEZ E, VÁZQUEZ JM, MATÁS C, GADEA J, ALONSO MI, ROCA J. Oocyte penetration by fresh or stored diluted boar spermatozoa before and after in vitro capacitation treatments. Biology of Reproduction 55: 134-139. 1996.
MARTÍNEZ E, VÁZQUEZ JM, MATÁS C, ROCA J. Are washing and preincubation of boar spermatozoa really necessary to penetrate pig oocytes under in vitro conditions? Reprod Dom Anim 31:317-320. 1996.
MATÁS C, MARTÍNEZ E, VÁZQUEZ JM, ROCA J, GADEA J. In vitro penetration assay of boar sperm fertility: Effect of various factors on the penetrability of immature pig oocytes. Theriogenology 46: 503-513. 1996.
VÁZQUEZ JM, MARTÍNEZ E, PASTOR LM, ROCA J, MATÁS C, CALVO A. Lectin histochemistry during "in vitro" capacitation and acrosome reaction in boar spermatozoa: new lectins for evaluating acrosomal status of boar spermatozoa. Acta Histochem 96: 93-100. 1996.
COY P, MARTÍNEZ E, RUIZ S, VÁZQUEZ JM, ROCA J, GADEA J. Environment and medium volume influence in vitro fertilisation of pig oocytes. Zygote 1: 209-213. 1993.
COY P, MARTÍNEZ E, RUIZ S, VÁZQUEZ JM, ROCA J, MATÁS C, PELLICER MT. In vitro fertilization of pig oocytes after different coincubation intervals. Theriogenology 39: 1201-1208.1993.
COY P, MARTÍNEZ E, RUIZ S, VÁZQUEZ JM, ROCA J, MATÁS C. Sperm concentration influences fertilization and male pronuclear formation in vitro in pigs. Theriogenology 40: 539-546. 1993.
MARTÍNEZ E, VÁZQUEZ JM, MATÁS C, ROCA J, COY P, GADEA J. Evaluation of boar spermatozoa penetrating capacity using pig oocytes at the germinal vesicle stage. Theriogenology 40: 547-557. 1993.
VÁZQUEZ JM, MARTÍNEZ E, ROCA J, COY P, PASTOR LM. Acrosome reaction of boar spermatozoa in homologous in vitro fertilization. Mol Reprod Dev 36:84-88. 1993.
MARTÍNEZ E, RUIZ S, ROCA J, VÁZQUEZ JM. Use of real-time ultrasonic scanning for the detection of reproductive failure in pig herds. Anim Reprod Sci 29:53-59. 1992.
ROCA J, MARTÍNEZ E, Sánchez-Valverde MA, VÁZQUEZ JM, RUIZ S. Seasonal variations of semen quality in male goats. Study of sperm abnormalities. Theriogenology 38:115-125. 1992
ROCA J, MARTÍNEZ E, VÁZQUEZ JM, COY P. Characteristics and seasonal variations in the semen of Murciano-granadina goats in the Mediterranean area. Anim Reprod Sci 29:255-262. 1992.
VÁZQUEZ JM, MARTÍNEZ E, ROCA J, COY P, RUIZ S. Use of triple stain technique for simultaneous assessment of vitality and acrosomal status in boar spermatozoa. Theriogenology 38:843-852. 1992.
ROCA J, MARTÍNEZ E, VÁZQUEZ JM, RUIZ S, COY P. Influence of season and photoperiod on testicular size and libido in male goats from the Mediterranean area. Anim Prod 93:10-14. 1991.

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